Abstract
Fluorescence energy transfer measurements have been performed to investigate the distances between the FITC-binding site in the (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum and 8-aniline-1-naftalenesulfonate (ANS-) and diphenylhexatriene (DPH) localized in different sites of the membrane. The distances calculated between FITC bound to the ATPase and ANS- and DPH in the membrane, were approx. 51 A and 60 A, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anilino Naphthalenesulfonates / metabolism*
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Animals
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Binding Sites
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Ca(2+) Mg(2+)-ATPase / metabolism*
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Calcium-Transporting ATPases / metabolism*
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Fluorescein-5-isothiocyanate
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Fluoresceins / metabolism*
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Fluorescent Dyes / metabolism*
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Kinetics
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Muscles / enzymology
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Protein Binding
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Rabbits
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Sarcoplasmic Reticulum / enzymology*
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Spectrometry, Fluorescence
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Thiocyanates / metabolism*
Substances
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Anilino Naphthalenesulfonates
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Fluoresceins
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Fluorescent Dyes
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Thiocyanates
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1-anilino-8-naphthalenesulfonate
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Ca(2+) Mg(2+)-ATPase
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Calcium-Transporting ATPases
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Fluorescein-5-isothiocyanate