Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

Charles Karavina; Jacques D Ibaba; Augustine Gubba

doi:10.1128/genomeA.01457-17

Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

Genome Announc. 2018 Mar 15;6(11):e01457-17. doi: 10.1128/genomeA.01457-17.

Authors

Charles Karavina^{1

2}, Jacques D Ibaba³, Augustine Gubba⁴

Affiliations

¹ Department of Plant Pathology, University of KwaZulu-Natal, Pietermaritzburg, KwaZulu-Natal, South Africa ckaravina@gmail.com.
² Department of Crop Science, Bindura University of Science Education, Bindura, Zimbabwe.
³ Genomic Research Institute, Centre for Microbial Ecology and Genomics, University of Pretoria, Hatfield, Pretoria, South Africa.
⁴ Department of Plant Pathology, University of KwaZulu-Natal, Pietermaritzburg, KwaZulu-Natal, South Africa.

Abstract

A tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay. Next-generation sequencing and de novo assembly were subsequently performed to determine its genome sequence. The ToMV genome of the Zimbabwe isolate is the second to be reported in Africa.