Abstract
Using GST fusion protein tags is an attractive approach for protein immobilization. Here we report that pyrimidine-based small-molecule probes with a fluorophosphonate reactive group could specifically react with the tyrosine-111 residue of the Schistosoma japonicum GST (sjGST) tag, and these probes could rapidly and site-selectively immobilize sjGST fusion proteins while preserving their activities.
MeSH terms
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Animals
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Glass / chemistry
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Glutathione Transferase / chemistry*
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Helminth Proteins / chemistry*
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Humans
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / chemistry
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Molecular Docking Simulation
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Molecular Probes / chemical synthesis
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Molecular Probes / chemistry*
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Organofluorophosphonates / chemical synthesis
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Organofluorophosphonates / chemistry*
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Pyrimidines / chemical synthesis
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Pyrimidines / chemistry
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Recombinant Fusion Proteins / chemistry
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Schistosoma japonicum / enzymology
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Sepharose / chemistry
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Tyrosine / chemistry
Substances
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Helminth Proteins
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Molecular Probes
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Organofluorophosphonates
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Pyrimidines
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Recombinant Fusion Proteins
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Tyrosine
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Sepharose
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Glutathione Transferase
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)