An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug-Protein Binding

Zackary M Herbst; Sayaka Shibata; Erkang Fan; Michael H Gelb

doi:10.1021/acsmedchemlett.7b00316

An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug-Protein Binding

ACS Med Chem Lett. 2017 Sep 28;9(3):279-282. doi: 10.1021/acsmedchemlett.7b00316. eCollection 2018 Mar 8.

Authors

Zackary M Herbst¹, Sayaka Shibata², Erkang Fan², Michael H Gelb^{3

2}

Affiliations

¹ Division of Allergy and Infectious Disease, Department of Medicine, University of Washington, Seattle, Washington 98195, United States.
² Department of Biochemistry, University of Washington, Seattle, Washington 98195, United States.
³ Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.

Abstract

An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fraction of the cost of a commercial, multichamber microdialysis device. Drug-protein binding results are provided, which validates the device.