A novel iridium(iii) complex for sensitive HSA phosphorescence staining in proteome research

Yi Wang; Huaiyi Huang; Ge Chen; Haijie Chen; Tingting Xu; Qian Tang; Hailiang Zhu; Qianling Zhang; Pingyu Zhang

doi:10.1039/c8cc01597j

A novel iridium(iii) complex for sensitive HSA phosphorescence staining in proteome research

Chem Commun (Camb). 2018 Mar 27;54(26):3282-3285. doi: 10.1039/c8cc01597j.

Authors

Yi Wang¹, Huaiyi Huang, Ge Chen, Haijie Chen, Tingting Xu, Qian Tang, Hailiang Zhu, Qianling Zhang, Pingyu Zhang

Affiliation

¹ College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, P. R. China. p.zhang6@szu.edu.cn.

PMID: 29537015
DOI: 10.1039/c8cc01597j

Abstract

We report a novel organo-iridium(iii) complex (Ir1) for the selective and sensitive determination of human serum albumin (HSA). The phosphorescence of Ir1 is turned-on by HSA in the urinary system. Importantly, Ir1 is much simpler and less time-consuming for HSA staining than Coomassie blue in proteomics studies.

MeSH terms

Coordination Complexes / chemistry*
Coordination Complexes / metabolism
Crystallography, X-Ray
Electrophoresis, Polyacrylamide Gel
Humans
Iridium / chemistry*
Molecular Conformation
Proteomics
Rosaniline Dyes / chemistry
Serum Albumin, Human / chemistry*
Serum Albumin, Human / metabolism
Serum Albumin, Human / urine
Spectrometry, Fluorescence

Substances

Coordination Complexes
Rosaniline Dyes
Iridium
Coomassie blue
Serum Albumin, Human