Objective: To develop a dynamic automatic identification system (device) of Schistosoma japonicum miracidia to achieve the automatic detection and improve the detection rate and efficiency of schistosome miracidia.
Methods: The dynamic automatic identification system (device) of S.japonicum miracidia was composed of an optical stereoscope, a digital camera, dynamic automatic tracking recognition software, and a computer. Under different conditions, the detection rates and efficiency of the system were compared with those of five professional persons.
Results: The basic function of the system was to identify, label and warn the miracidia of S. japonicum, and the records could be saved automatically. The laboratory tests showed that the missing rate of the system was 0. The total consistent rates of the manual detection were 74.67% and 66.67% in the condition with and without water bug, while the total consistent rates of the system were 100.00% and 96.67%, respectively (χ2 = 9.634, 11.081, both P < 0.01) .
Conclusions: The system is much superior to manual detection in the accuracy and speed, and the system could completely replace the manual detection. Therefore, the system could be used in the field and basic research of schistosomiasis.
［摘要］目的 研发血吸虫毛蚴动态自动识别系统 (装置), 实现其自动化检测, 以提高其检测效率和检出率。 方法 血 吸虫毛蚴动态自动识别系统 (装置) 由光学体视镜、数字摄像机、动态自跟踪识别软件和计算机组成。在不同条件下, 对 比系统检测与5名专业人员人工检测的效率、准确率等。 结果 血吸虫毛蚴动态自动识别系统 (装置) 的基本功能为毛 蚴动态识别、标记、提醒, 并可以自动保存视频和生成登记表、检验单等。室内实验该系统可自动识别毛蚴, 无漏检率。人工检测无水虫组总符合率为74.67%, 有水虫组为66.67%, 系统检测总符合率则分别为100.00%和96.67%, 差异有统计 学意义 (χ2 = 9.634、11.081, P 均< 0.01), 系统检测效果优于人工。 结论 血吸虫毛蚴动态自动识别系统 (装置) 可以替代 人工观察, 该系统可以用于血吸虫病的现场查病及相关基础研究。.
Keywords: Automatic identification; Effectiveness evaluation; Miracidium; Schistosoma japonicum.