Combination of resveratrol-containing collagen with adipose stem cells for craniofacial tissue-engineering applications

Chih-Chien Wang; Chih-Hsin Wang; Hsiang-Cheng Chen; Juin-Hong Cherng; Shu-Jen Chang; Yi-Wen Wang; Adrienne Chang; Jue-Zong Yeh; Yi-Huei Huang; Cheng-Che Liu

doi:10.1111/iwj.12910

Combination of resveratrol-containing collagen with adipose stem cells for craniofacial tissue-engineering applications

Int Wound J. 2018 Aug;15(4):660-672. doi: 10.1111/iwj.12910. Epub 2018 Mar 13.

Authors

Chih-Chien Wang¹, Chih-Hsin Wang², Hsiang-Cheng Chen³, Juin-Hong Cherng^{4

5

6}, Shu-Jen Chang³, Yi-Wen Wang⁴, Adrienne Chang⁷, Jue-Zong Yeh⁸, Yi-Huei Huang⁹, Cheng-Che Liu¹⁰

Affiliations

¹ Department of Orthopedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
² Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
³ Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
⁴ Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
⁵ General Clinical Research Center for New Drug Trial, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
⁶ Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan (R.O.C.).
⁷ Department of Chemistry, New York University, Abu Dhabi, United Arab Emirates.
⁸ Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan (R.O.C.).
⁹ Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan (R.O.C.).
¹⁰ Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei, Taiwan (R.O.C.).

Abstract

Repair and regeneration of craniofacial tissues is particularly challenging because they comprise a complex structure of hard and soft tissues involved in intricate functions. This study combined collagen scaffolds and human adipose stem cells (hASCs) for oral mucosal and calvarial bone regeneration by using resveratrol (RSV), which affects the differentiation of mesenchymal stem cells. We have evaluated the effect of collagen scaffold-containing RSV (collagen/RSV) scaffolds both in vitro and in vivo for their wound healing and bone regeneration potential. Scanning electron microscopy and immunostaining results reveal that hASCs adhere well to and proliferate on both collagen scaffolds and collagen/RSV scaffolds. Oral mucosal lesion experiments demonstrated that the collagen/RSV scaffold is more effective in wound closure and contraction than the collagen scaffold. The micro-computed tomography (μCT) images of calvarial bone display regenerating bone in defects covered with hASCs on collagen/RSV scaffolds that are more visible than that in defects covered with hASCs on a collagen scaffolds. RSV was more effective at inducing hASC differentiation on the collagen scaffold, suggesting that collagen/RSV scaffolds can provide useful biological cues that stimulate craniofacial tissue formation.

Keywords: biocompatibility; bone regeneration; collagen; oral mucosal; resveratrol.

MeSH terms

Adipose Tissue / transplantation*
Animals
Cell Proliferation / physiology*
Cells, Cultured / physiology
Collagen / therapeutic use*
Craniofacial Abnormalities / surgery*
Humans
Models, Animal
Rats
Resveratrol / therapeutic use*
Stem Cell Transplantation / methods*
Tissue Engineering / methods*
Tissue Scaffolds

Substances

Collagen
Resveratrol