Abstract
Quantification of single-cell proteomics provides key insights in the field of cellular heterogeneity. This chapter discusses the emerging techniques that are being used to measure the protein copy numbers at the single-cell level, which includes flow cytometry, mass cytometry, droplet cytometry, microengraving, and single-cell barcoding microchip. The advantages and limitations of each technique are compared, and future research opportunities are highlighted.
Keywords:
Droplet cytometry; Flow cytometry; Mass cytometry; Microengraving; Single-cell barcoding microchip; Single-cell protein assays.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Humans
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Mass Spectrometry / instrumentation
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Mass Spectrometry / methods
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Microfluidic Analytical Techniques / instrumentation
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Microfluidic Analytical Techniques / methods
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Microtechnology / instrumentation
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Microtechnology / methods
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Protein Array Analysis / instrumentation
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Protein Array Analysis / methods
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Proteins / analysis*
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Proteins / metabolism
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Proteomics / instrumentation
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Proteomics / methods*
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Proteomics / trends
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Single-Cell Analysis / instrumentation
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Single-Cell Analysis / methods*