Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro

Fawaz Abomaray; Sebastian Gidlöf; Bartosz Bezubik; Mikael Engman; Cecilia Götherström

doi:10.1155/2018/7318513

Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro

Stem Cells Int. 2018 Jan 28:2018:7318513. doi: 10.1155/2018/7318513. eCollection 2018.

Authors

Fawaz Abomaray^{1

2}, Sebastian Gidlöf^{1

3

4}, Bartosz Bezubik⁵, Mikael Engman⁶, Cecilia Götherström^{1

2}

Affiliations

¹ Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
² Centre for Hematology and Regenerative Medicine, Karolinska Institutet, Stockholm, Sweden.
³ Center for Fetal Medicine, Patient Area of Pregnancy and Childbirth, Karolinska University Hospital, Stockholm, Sweden.
⁴ Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
⁵ Department of Obstetrics and Gynecology, Karolinska University Hospital, Stockholm, Sweden.
⁶ Department of Obstetrics and Gynecology, Danderyd's Hospital, Stockholm, Sweden.

Abstract

Endometriosis is an inflammatory disease marked by ectopic growth of endometrial cells. Mesenchymal stromal cells (MSC) have immunosuppressive properties that have been suggested as a treatment for inflammatory diseases. Therefore, the aim herein was to examine effects of allogeneic MSC on endometriosis-derived cells in vitro as a potential therapy for endometriosis. MSC from allogeneic adipose tissue (Ad-MSC) and stromal cells from endometrium (ESC_endo) and endometriotic ovarian cysts (ESC_cyst) from women with endometriosis were isolated. The effects of Ad-MSC on ESC_endo and ESC_cyst were investigated using in vitro proliferation, apoptosis, adhesion, tube formation, migration, and invasion assays. Ad-MSC significantly increased proliferation of ESC compared to untreated controls. Moreover, Ad-MSC significantly decreased apoptosis and increased survival of ESC. Ad-MSC significantly increased adhesion of ESC_endo and not ESC_cyst on fibronectin. Conditioned medium from cocultures of Ad-MSC and ESC significantly increased tube formation of human umbilical vein endothelial cells on matrigel. Ad-MSC may significantly increase migration of ESC_cyst and did not increase invasion of both cell types. The data suggest that allogeneic Ad-MSC should not be considered as a potential therapy for endometriosis, because they may support the pathology by maintaining and increasing growth of ectopic endometrial tissue.