Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication

Viruses. 2018 Mar 13;10(3):127. doi: 10.3390/v10030127.

Abstract

The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.

Keywords: heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1); nucleocapsid protein; porcine epidemic diarrhea virus (PEDV).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coronavirus Infections / veterinary*
  • Gene Knockdown Techniques
  • Heterogeneous Nuclear Ribonucleoprotein A1 / genetics
  • Heterogeneous Nuclear Ribonucleoprotein A1 / metabolism*
  • Humans
  • Jejunum / pathology
  • Jejunum / virology
  • Nucleocapsid / metabolism*
  • Porcine epidemic diarrhea virus / physiology*
  • Protein Binding
  • Protein Transport
  • Swine
  • Swine Diseases / metabolism*
  • Swine Diseases / virology*
  • Virus Replication*

Substances

  • Heterogeneous Nuclear Ribonucleoprotein A1