Plant postembryonic development takes place in region called meristems that represent a reserve of undifferentiated cells. In the root meristem of Arabidopsis thaliana, all tissues originate from a stem-cell niche. Stem-cell daughters undergo a finite number of cell divisions until they reach the transition zone where divisions cease and cells start to differentiate. For meristem maintenance, and therefore continuous root growth, the rate of cell differentiation must equal the rate of generation of new cells. How this balance is achieved is a central question in plant biology. In this chapter we described protocols to help the operator in approaching developmental studies on the Arabidopsis root meristem.
Keywords: Auxin; Cell differentiation; Cell division; Cytokinin; Meristem; Transition boundary.