Role of the potassium/lysine cationic center in catalysis and functional asymmetry in membrane-bound pyrophosphatases

Biochem J. 2018 Mar 26;475(6):1141-1158. doi: 10.1042/BCJ20180071.

Abstract

Membrane-bound pyrophosphatases (mPPases), which couple pyrophosphate hydrolysis to transmembrane transport of H+ and/or Na+ ions, are divided into K+,Na+-independent, Na+-regulated, and K+-dependent families. The first two families include H+-transporting mPPases (H+-PPases), whereas the last family comprises one Na+-transporting, two Na+- and H+-transporting subfamilies (Na+-PPases and Na+,H+-PPases, respectively), and three H+-transporting subfamilies. Earlier studies of the few available model mPPases suggested that K+ binds to a site located adjacent to the pyrophosphate-binding site, but is substituted by the ε-amino group of an evolutionarily acquired lysine residue in the K+-independent mPPases. Here, we performed a systematic analysis of the K+/Lys cationic center across all mPPase subfamilies. An Ala → Lys replacement in K+-dependent mPPases abolished the K+ dependence of hydrolysis and transport activities and decreased these activities close to the level (4-7%) observed for wild-type enzymes in the absence of monovalent cations. In contrast, a Lys → Ala replacement in K+,Na+-independent mPPases conferred partial K+ dependence on the enzyme by unmasking an otherwise conserved K+-binding site. Na+ could partially replace K+ as an activator of K+-dependent mPPases and the Lys → Ala variants of K+,Na+-independent mPPases. Finally, we found that all mPPases were inhibited by excess substrate, suggesting strong negative co-operativity of active site functioning in these homodimeric enzymes; moreover, the K+/Lys center was identified as part of the mechanism underlying this effect. These findings suggest that the mPPase homodimer possesses an asymmetry of active site performance that may be an ancient prototype of the rotational binding-change mechanism of F-type ATPases.

Keywords: allosteric regulation; cation transporter; enzyme kinetics; membrane-bound pyrophosphatase; protein evolution; pyrophosphate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Cations
  • Cell Membrane / metabolism*
  • Desulfitobacterium / enzymology
  • Desulfitobacterium / genetics
  • Escherichia coli
  • Geobacter / enzymology
  • Geobacter / genetics
  • Ion Transport / physiology
  • Lysine / metabolism*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Phylogeny
  • Potassium / metabolism*
  • Protein Multimerization*
  • Protein Structure, Quaternary
  • Pyrophosphatases / chemistry*
  • Pyrophosphatases / genetics
  • Pyrophosphatases / metabolism*

Substances

  • Cations
  • Membrane Proteins
  • Pyrophosphatases
  • Lysine
  • Potassium