Catch the live show: Visualizing damaged DNA in vivo

Methods. 2018 Jun 1:142:24-29. doi: 10.1016/j.ymeth.2018.02.023. Epub 2018 Mar 6.

Abstract

The health of an organism is intimately linked to its ability to repair damaged DNA. Importantly, DNA repair processes are highly dynamic. This highlights the necessity of characterizing DNA repair in live cells. Advanced genome editing and imaging approaches allow us to visualize damaged DNA and its associated factors in real time. Here, we summarize both established and recent methods that are used to induce DNA damage and visualize damaged DNA and its repair in live cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Chromatin / chemistry
  • Chromatin / genetics
  • Chromatin / metabolism
  • DNA / chemistry
  • DNA / genetics
  • DNA / metabolism*
  • DNA Damage / drug effects
  • DNA Damage / genetics*
  • DNA Damage / radiation effects
  • DNA Repair / genetics
  • Endonucleases / genetics
  • Endonucleases / metabolism
  • Humans
  • Intravital Microscopy / instrumentation
  • Intravital Microscopy / methods*
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / genetics
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Molecular Imaging / instrumentation
  • Molecular Imaging / methods*
  • Rad52 DNA Repair and Recombination Protein / chemistry
  • Rad52 DNA Repair and Recombination Protein / genetics
  • Rad52 DNA Repair and Recombination Protein / metabolism

Substances

  • Chromatin
  • Luminescent Proteins
  • Rad52 DNA Repair and Recombination Protein
  • DNA
  • Endonucleases