Detection of trace amounts of citrinin in dried orange peel by using an optimized extraction method coupled with ultra-performance liquid chromatography-tandem mass spectrometry

Biomed Chromatogr. 2018 Aug;32(8):e4237. doi: 10.1002/bmc.4237. Epub 2018 Apr 6.

Abstract

A fast and sensitive method involving ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was introduced to detect citrinin in dried orange peel. A series of extraction, purification and chromatographic conditions was also systematically examined. With the proposed method, the obtained calibration graph was linear, with an R of 0.9996 within a concentration range of 0.5-10 ng/mL. The estimated limits of detection and quantification were 0.05 and 0.17 ng/mL, respectively. Under the selected conditions, the relative recoveries in different citrus products spiked with 1-10 ng/mL citrinin were 89.4-98.7% with RSDs of <2.5%. Compared with previously reported analytical methods, the newly developed UPLC-MS/MS method showed excellent sensitivity and good precision in detecting citrinin. The results indicated that it is a reliable and effective technique for the detection of trace citrinin in dried orange peel.

Keywords: UPLC-MS/MS; citrinin; dried orange peel; extraction.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Citrinin / analysis*
  • Citrinin / chemistry
  • Citrinin / isolation & purification
  • Citrus sinensis / chemistry*
  • Linear Models
  • Pesticide Residues / analysis*
  • Pesticide Residues / chemistry
  • Pesticide Residues / isolation & purification
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Solid Phase Extraction
  • Tandem Mass Spectrometry / methods*

Substances

  • Pesticide Residues
  • Citrinin