Emergence of Different Recombinant Porcine Reproductive and Respiratory Syndrome Viruses, China

Yanyan Liu; Jianda Li; Jie Yang; Hao Zeng; Lihui Guo; Sufang Ren; Wenbo Sun; Zhi Chen; Xiaoyan Cong; Jianli Shi; Lei Chen; Yijun Du; Jun Li; Jinbao Wang; Jiaqiang Wu; Jiang Yu

doi:10.1038/s41598-018-22494-4

Emergence of Different Recombinant Porcine Reproductive and Respiratory Syndrome Viruses, China

Sci Rep. 2018 Mar 7;8(1):4118. doi: 10.1038/s41598-018-22494-4.

Authors

Yanyan Liu^{1

2

3}, Jianda Li¹, Jie Yang¹, Hao Zeng¹, Lihui Guo¹, Sufang Ren¹, Wenbo Sun¹, Zhi Chen¹, Xiaoyan Cong¹, Jianli Shi¹, Lei Chen¹, Yijun Du^{1

4}, Jun Li^{1

4}, Jinbao Wang^{5

6

7}, Jiaqiang Wu^{8

9

10}, Jiang Yu¹¹

Affiliations

¹ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
² School of Life Sciences, Shandong University, Jinan, 250100, China.
³ Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
⁴ School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China.
⁵ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. wangjb@saas.ac.cn.
⁶ School of Life Sciences, Shandong University, Jinan, 250100, China. wangjb@saas.ac.cn.
⁷ School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China. wangjb@saas.ac.cn.
⁸ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. wujiaqiang2000@sina.com.
⁹ School of Life Sciences, Shandong University, Jinan, 250100, China. wujiaqiang2000@sina.com.
¹⁰ School of Life Sciences, Shandong Normal University, Jinan, Jinan, 250014, China. wujiaqiang2000@sina.com.
¹¹ Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. yujiang_2213@163.com.

Abstract

Epidemiological investigations were conducted on recently emerging porcine reproductive and respiratory syndrome virus (PRRSV) strains in Shandong province in 2014-2015. The proportion of the NADC30 strain identified by ORF7 sequence alignment has been gradually increasing. Three emerging PRRSV strains were successfully isolated, and the complete genomic sequences were determined. Our results indicate the importance of recombinant strains in Shandong province, China. There was a varied degree of recombination of two or three strains (classical, HP-PRRSV and/or NADC30). Moreover, the recombination strains affected the pathogenicity of newly emerged strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome, Viral*
Phylogeny*
Porcine Reproductive and Respiratory Syndrome / genetics*
Porcine respiratory and reproductive syndrome virus / genetics*
Porcine respiratory and reproductive syndrome virus / isolation & purification
Recombination, Genetic*
Sequence Alignment
Swine / virology*