Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens

Curr Protoc Toxicol. 2018 Feb 21:75:20.15.1-20.15.20. doi: 10.1002/cptx.44.

Abstract

Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE).© 2018 by John Wiley & Sons, Inc.

Keywords: 3D model; contact allergens; contact hypersensitivity; in vitro; potency; reconstituted human epidermis.

MeSH terms

  • Allergens / toxicity*
  • Dermatitis, Contact / etiology
  • Epidermis / drug effects*
  • Humans
  • In Vitro Techniques
  • Interleukin-18
  • Irritants / toxicity*
  • Skin Irritancy Tests / methods*

Substances

  • Allergens
  • Interleukin-18
  • Irritants