Hepatitis E Virus: Isolation, Propagation, and Quantification

Curr Protoc Microbiol. 2018 Feb 22:48:15L.1.1-15L.1.15. doi: 10.1002/cpmc.50.

Abstract

Hepatitis E virus (HEV) predominantly causes acute liver disease in humans and is transmitted via the fecal-oral route. HEV infection in pregnant women can result in grave consequences, with up to 30% fatality. The HEV strains infecting humans mainly belong to four genotypes. Genotypes 1 and 2 are restricted to human infection, while genotypes 3 and 4 are zoonotic. HEV genotype 3 (HEV-3) can cause both acute and chronic liver diseases. Several cell lines (mainly hepatocytes) have been developed for HEV propagation and biological study. However, HEV production in these cell lines is suboptimal and inefficient. Here, we present methods for the isolation, propagation, and quantification of HEV. © 2018 by John Wiley & Sons, Inc.

Keywords: HEV; isolation; propagation; virus quantification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Feces / virology
  • Hepatitis E / virology*
  • Hepatitis E virus / classification
  • Hepatitis E virus / genetics
  • Hepatitis E virus / growth & development
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Virus Cultivation / methods*