Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification

Curr Protoc Microbiol. 2018 Feb 22:48:15M.1.1-15M.1.14. doi: 10.1002/cpmc.51.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirale. PRRSV is an enveloped, single-stranded, positive-sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC-145 cell line is the most often used in a laboratory setting. Infectious cDNA clones of many PRRSV strains have been established, from which these viruses can be recovered. PRRSV titration is generally done in MARC-145 cells. PRRSV RNA copy numbers can be assessed by reverse transcription and real-time PCR. Here, protocols for PRRSV propagation, virus recovery from infectious cDNA clones, and quantification are presented. © 2018 by John Wiley & Sons, Inc.

Keywords: PRRSV; infectious clone; propagation; quantification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Line
  • Polymerase Chain Reaction / methods*
  • Porcine Reproductive and Respiratory Syndrome / virology*
  • Porcine respiratory and reproductive syndrome virus / genetics
  • Porcine respiratory and reproductive syndrome virus / growth & development*
  • Porcine respiratory and reproductive syndrome virus / physiology
  • Swine
  • Virus Cultivation / methods*
  • Virus Replication