To study the effect of quality nursing on postoperative incision infection in urological patients, 200 subjects admitted to our hospital between June 2016 and June 2017 were included in this study and divided into a quality nursing group (group A) and a general nursing group (group B), 100 in each group. Blood loss, blood transfusion, hospital stay, incision healing, incision infection, and self-rating depression scale (SDS) scores in both groups were compared. It was found that the bleeding volume in group A was significantly less than that in group B, and there was significant difference between the two groups (P less than 0.05) while the difference in blood transfusion rate and hospital stay between the two groups was not significant (P> 0.05); the number of patients of level 1 healing in group A was larger than that of group B while the number of patients of level 2 and level 3 healing was smaller than that of group B, with significant differences (P less than 0.05); the number of infection cases in group A was significantly lower than that in group B, and the difference was significant (P less than 0.05); the SDS score of group A was lower than that of group B, with significant differences (P less than 0.05). Therefore, quality nursing had a certain effect on the infection of postoperative incision of urological patients, which had positive significance for incision healing. Reducing the effect of bacterial infections in operated patients is important for rapid healing and patient health. Using a long-lasting antibacterial can protect the patient and reduce the incidence of other infections.