Abstract
The results of a complex morphofunctional study with markers of proliferation (PCNA and Ki-67), angiogenesis (CD31 and CD34), and structural integrity of mesenchymal cells (vimentin) suggest that the pool of primordial follicles was well preserved in vitrified ovarian tissue and that viability of its cellular components can be rapidly restored during incubation at 37°C within 4 h after thawing.
Keywords:
follicles; immunohistochemistry; ovarian tissue; vitrification.
MeSH terms
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Adult
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Antigens, CD34 / genetics
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Antigens, CD34 / metabolism
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Biomarkers / metabolism
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Cell Survival
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Cryopreservation / methods*
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Cryoprotective Agents / pharmacology
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Culdoscopy
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Female
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Gene Expression
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Humans
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Ki-67 Antigen / genetics
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Ki-67 Antigen / metabolism
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Middle Aged
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Ovarian Follicle / cytology*
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Ovarian Follicle / drug effects
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Ovarian Follicle / metabolism
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Ovary / cytology*
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Ovary / drug effects
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Ovary / metabolism
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Platelet Endothelial Cell Adhesion Molecule-1 / genetics
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Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
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Proliferating Cell Nuclear Antigen / genetics
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Proliferating Cell Nuclear Antigen / metabolism
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Tissue Culture Techniques
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Vimentin / genetics
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Vimentin / metabolism
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Vitrification*
Substances
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Antigens, CD34
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Biomarkers
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Cryoprotective Agents
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Ki-67 Antigen
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Platelet Endothelial Cell Adhesion Molecule-1
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Proliferating Cell Nuclear Antigen
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Vimentin