Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries

А D Voronova; O V Stepanova; M P Valikhov; A V Chadin; А S Dvornikov; I V Reshetov; V P Chekhonin

doi:10.1007/s10517-018-4025-x

Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries

Bull Exp Biol Med. 2018 Mar;164(4):523-527. doi: 10.1007/s10517-018-4025-x. Epub 2018 Mar 5.

Authors

А D Voronova^{1

2}, O V Stepanova³, M P Valikhov³, A V Chadin³, А S Dvornikov³, I V Reshetov⁴, V P Chekhonin^{5

3}

Affiliations

¹ Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia. nastyanastyav@mail.ru.
² Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia. nastyanastyav@mail.ru.
³ Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.
⁴ University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
⁵ Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.

PMID: 29504110
DOI: 10.1007/s10517-018-4025-x

Abstract

We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.

Keywords: cell therapy; human olfactory mucosa; olfactory ensheathing cells from human olfactory mucosa; spinal cord injuries.

MeSH terms

Biomarkers / metabolism
Cell Proliferation
Cell- and Tissue-Based Therapy / methods*
Gene Expression
Glial Fibrillary Acidic Protein / genetics
Glial Fibrillary Acidic Protein / metabolism
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Olfactory Bulb / cytology*
Olfactory Bulb / metabolism
Olfactory Bulb / surgery
Olfactory Mucosa / cytology*
Olfactory Mucosa / metabolism
Olfactory Mucosa / surgery
Primary Cell Culture / methods*
Receptors, Nerve Growth Factor / genetics
Receptors, Nerve Growth Factor / metabolism
Spinal Cord Injuries / therapy

Substances

Biomarkers
Glial Fibrillary Acidic Protein
NGFR protein, human
Nerve Tissue Proteins
Receptors, Nerve Growth Factor