Monocytes with Oncogenic Mutation JAK2 V617F as a Tool for Studies of the Pathogenic Mechanisms of Myelofibrosis

A A Silyutina; I I Gin; S S Prikhod'ko; S V Zhuk; P A Butylin; A Yu Zaritskii

doi:10.1007/s10517-018-4033-x

Monocytes with Oncogenic Mutation JAK2 V617F as a Tool for Studies of the Pathogenic Mechanisms of Myelofibrosis

Bull Exp Biol Med. 2018 Mar;164(4):569-575. doi: 10.1007/s10517-018-4033-x. Epub 2018 Mar 5.

Authors

A A Silyutina¹, I I Gin², S S Prikhod'ko², S V Zhuk², P A Butylin², A Yu Zaritskii²

Affiliations

¹ Research Laboratory of Oncohematology, Institute of Hematology, V. A. Almazov National Medical Research Centre, St. Petersburg, Russia. silyutina.anna89@gmail.com.
² Research Laboratory of Oncohematology, Institute of Hematology, V. A. Almazov National Medical Research Centre, St. Petersburg, Russia.

PMID: 29504105
DOI: 10.1007/s10517-018-4033-x

Abstract

We analyzed previously generated stable monocyte-derived cell line carrying mutation JAK2 V617F. Evaluation of the expression of pro- and antifibrotic factors revealed changes in the production of MMPs and their inhibitors, growth factors, galectin-3, and pentraxin 3 in cells carrying mutation JAK2 in comparison with control non-modified cells.

Keywords: JAK2 V617F; macrophages; myeloproliferative neoplasms; primary myelofibrosis.

MeSH terms

Blood Proteins
C-Reactive Protein / genetics
C-Reactive Protein / metabolism
Cell Communication
Coculture Techniques
Galectin 3 / genetics
Galectin 3 / metabolism
Galectins
Gene Expression Regulation*
Humans
Janus Kinase 2 / genetics*
Janus Kinase 2 / metabolism
Matrix Metalloproteinases / genetics
Matrix Metalloproteinases / metabolism
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism*
Models, Biological
Mutation*
Primary Cell Culture
Primary Myelofibrosis / genetics
Primary Myelofibrosis / metabolism
Primary Myelofibrosis / pathology
Protein Isoforms / genetics
Protein Isoforms / metabolism
Serum Amyloid P-Component / genetics
Serum Amyloid P-Component / metabolism
Signal Transduction
THP-1 Cells
Tissue Inhibitor of Metalloproteinases / genetics
Tissue Inhibitor of Metalloproteinases / metabolism

Substances

Blood Proteins
Galectin 3
Galectins
LGALS3 protein, human
Protein Isoforms
Serum Amyloid P-Component
Tissue Inhibitor of Metalloproteinases
PTX3 protein
C-Reactive Protein
JAK2 protein, human
Janus Kinase 2
Matrix Metalloproteinases