Purification and identification of anti-inflammatory peptides from spent hen muscle proteins hydrolysate

Food Chem. 2018 Jul 1:253:101-107. doi: 10.1016/j.foodchem.2018.01.093. Epub 2018 Jan 12.

Abstract

Spent hens have low market value and incur significant costs for disposal. This study explored the use of spent hens as a source of bioactive peptides. Spent hen hydrolysates prepared by Protease M or Protex 50FP exhibited interleukin (IL)-6 inhibitory activity in endotoxin-activated macrophage-like U937 cells (p < .05). The potential peptides from Protex 50FP hydrolysate were further fractionated using a combination of ultrafiltration, solid-phase extraction and high-performance liquid chromatography; 17 novel peptides encoded in major muscle proteins were identified by mass spectrometry analysis, of which 7 were chemically synthesized and assayed for the IL-6 inhibitory activity. At a concentration of 100 µg/mL, peptide FLWGKSY induced a 79% reduction of IL-6 production in endotoxin-activated macrophage-like U937 cells, which is comparable to results reported from other food sources. Our results indicate that spent hens have potential to be a source of bioactive peptides for anti-inflammatory applications.

Keywords: Anti-inflammatory peptides; Identification; Mass spectrometry; Purification; Spent hen.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification*
  • Anti-Inflammatory Agents / pharmacology
  • Chickens / metabolism*
  • Chromatography, High Pressure Liquid / veterinary
  • Female
  • Humans
  • Interleukin-6 / antagonists & inhibitors*
  • Mass Spectrometry / veterinary
  • Muscle Proteins / chemistry*
  • Muscle Proteins / isolation & purification
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Peptides / pharmacology
  • Protein Hydrolysates / chemistry*
  • Protein Hydrolysates / isolation & purification
  • Solid Phase Extraction / veterinary
  • U937 Cells

Substances

  • Anti-Inflammatory Agents
  • IL6 protein, human
  • Interleukin-6
  • Muscle Proteins
  • Peptides
  • Protein Hydrolysates