RGD-Functionalized Fe3O4 nanoparticles for magnetic hyperthermia

Oihane K Arriortua; Maite Insausti; Luis Lezama; Izaskun Gil de Muro; Eneko Garaio; Jesus Martínez de la Fuente; Raluca M Fratila; Maria P Morales; Rocío Costa; Maite Eceiza; Maialen Sagartzazu-Aizpurua; Jesus M Aizpurua

doi:10.1016/j.colsurfb.2018.02.031

RGD-Functionalized Fe₃O₄ nanoparticles for magnetic hyperthermia

Colloids Surf B Biointerfaces. 2018 May 1:165:315-324. doi: 10.1016/j.colsurfb.2018.02.031. Epub 2018 Feb 15.

Affiliations

¹ BC Materials, Basque Center for Materials, Applications & Nanostructures, Spain.
² BC Materials, Basque Center for Materials, Applications & Nanostructures, Spain; Inorganic Chemistry, Department University of Basque Country, 48940, Leioa, Spain. Electronic address: maite.insausti@ehu.es.
³ BC Materials, Basque Center for Materials, Applications & Nanostructures, Spain; Inorganic Chemistry, Department University of Basque Country, 48940, Leioa, Spain.
⁴ Electricity and Electronic Department, University of Basque Country, 48940, Leioa, Spain.
⁵ Group of Biofunctional Nanoparticles and Surfaces, Instituto de Ciencia de Materiales de Aragón, CSIC/University of Zaragoza and CIBER-BBN. Zaragoza, Spain.
⁶ Biomaterials and Bioinspired Materials Department, Instituto de Ciencia de Materiales de Madrid, ICMM-CSIC, 28049, Madrid, Spain.
⁷ José Mari Korta R&D Center, Basque Country University, UPV/EHU, 20018, Donostia, Spain.

PMID: 29501962
DOI: 10.1016/j.colsurfb.2018.02.031

Abstract

To improve the selectivity of magnetic nanoparticles for tumor treatment by hyperthermia, Fe₃O₄ nanoparticles have been functionalized with a peptide of the type arginine-glycine-aspartate (RGD) following a "click" chemistry approach. The RGD peptide was linked onto the previously coated nanoparticles in order to target α_vβ₃ integrin receptors over-expressed in angiogenic cancer cells. Different coatings have been analyzed to enhance the biocompatibility of magnetic nanoparticles. Monodispersed and homogeneous magnetite nanoparticles have been synthesized by the seed growth method and have been characterized using X-ray diffraction, thermogravimetric analysis, infrared spectroscopy, transmission electron microscopy and magnetic measurements. The magnetic hyperthermia efficiency of the nanoparticles has also been investigated and cytotoxicity assays have been perfomed for functionalized nanoparticles.

Keywords: Citotoxicity; EMR; Hyperthermia; Magnetite; RGD.

MeSH terms

Animals
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Cell Line, Tumor
Cell Survival / drug effects
Chlorocebus aethiops
Ferrosoferric Oxide / chemistry*
Gene Expression
Humans
Hyperthermia, Induced*
Integrin alphaVbeta3 / genetics
Integrin alphaVbeta3 / metabolism*
Magnetite Nanoparticles / administration & dosage*
Magnetite Nanoparticles / chemistry
Magnetite Nanoparticles / ultrastructure
Oligopeptides / chemistry*
Protein Binding
Vero Cells

Substances

Biomarkers, Tumor
Integrin alphaVbeta3
Magnetite Nanoparticles
Oligopeptides
arginyl-glycyl-aspartic acid
Ferrosoferric Oxide