Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor

Methods. 2018 May 1:140-141:74-84. doi: 10.1016/j.ymeth.2018.01.010. Epub 2018 Mar 1.

Abstract

Image correlation analysis has evolved to become a valuable method of analysis of the diffusional motion of molecules in every points of a live cell. Here we compare the iMSD and the 2D-pCF approaches that provide complementary information. The iMSD method provides the law of diffusion and it requires spatial averaging over a small region of the cell. The 2D-pCF does not require spatial averaging and it gives information about obstacles for diffusion at pixel resolution. We show the analysis of the same set of data by the two methods to emphasize that both methods could be needed to have a comprehensive understanding of the molecular diffusional flow in a live cell.

Keywords: Barrier to diffusion; Connectivity maps; Diffusion anisotropy; Fluorescence fluctuation spectroscopy.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • CHO Cells
  • Cricetulus
  • Diffusion
  • ErbB Receptors / chemistry
  • ErbB Receptors / metabolism
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / metabolism
  • Image Processing, Computer-Assisted / methods*
  • Intravital Microscopy / instrumentation
  • Intravital Microscopy / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Models, Chemical*

Substances

  • Green Fluorescent Proteins
  • EGFR protein, human
  • ErbB Receptors