A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems

Xuhui Geng; Meng Shi; Haijing Ning; Chunbo Feng; Yafeng Guan

doi:10.1016/j.talanta.2018.01.076

A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems

Talanta. 2018 May 15:182:279-284. doi: 10.1016/j.talanta.2018.01.076. Epub 2018 Feb 20.

Authors

Xuhui Geng¹, Meng Shi², Haijing Ning¹, Chunbo Feng¹, Yafeng Guan³

Affiliations

¹ Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
² Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
³ Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. Electronic address: guanyafeng@dicp.ac.cn.

PMID: 29501153
DOI: 10.1016/j.talanta.2018.01.076

Abstract

A compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 × 10^-12 M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them.

Keywords: Compact; Laser induced fluorescence; Low-cost; Single cell.