Isolation and Purification of Two Isoflavones from Hericium erinaceum Mycelium by High-Speed Counter-Current Chromatography

Molecules. 2018 Mar 2;23(3):560. doi: 10.3390/molecules23030560.

Abstract

High-speed counter-current chromatography (HSCCC) was used to separate and purify two isoflavones for the first time from Hericium erinaceum (H. erinaceum) mycelium using a two-phase solvent system composed of chloroform-dichloromethane-methanol-water (4:2:3:2, v/v/v/v). These two isoflavones were identified as genistein (4',5,7-trihydroxyisoflavone, C15H10O₅) and daidzein (4',7-dihydroxyisoflavone, C15H10O₄), using infrared spectroscopy (IR), electro-spary ionisation mass (ESI-MS), ¹H-nuclear magnetic resonance (NMR) and 13C-NMR spectra. About 23 mg genistein with 95.7% purity and 18 mg daidzein with 97.3% purity were isolated from 150 mg ethanolic extract of H. erinaceum mycelium. The results demonstrated that HSCCC was a feasible method to separate and purify genistein and daidzein from H. erinaceum mycelium.

Keywords: Hericium erinaceuns mycelium; daidzein; genistein; high-speed counter-current chromatography (HSCCC).

MeSH terms

  • Agaricales / chemistry*
  • Countercurrent Distribution / methods
  • Genistein / isolation & purification*
  • Isoflavones / isolation & purification*
  • Liquid-Liquid Extraction / methods
  • Mycelium / chemistry*
  • Solvents / chemistry

Substances

  • Isoflavones
  • Solvents
  • daidzein
  • Genistein