3D imaging in the postmortem human brain with CLARITY and CUBIC

Kunie Ando; Quentin Laborde; Jean-Pierre Brion; Charles Duyckaerts

doi:10.1016/B978-0-444-63639-3.00021-9

3D imaging in the postmortem human brain with CLARITY and CUBIC

Handb Clin Neurol. 2018:150:303-317. doi: 10.1016/B978-0-444-63639-3.00021-9.

Authors

Kunie Ando¹, Quentin Laborde², Jean-Pierre Brion³, Charles Duyckaerts²

Affiliations

¹ Laboratory of Histology, Neuroanatomy and Neuropathology, ULB Neuroscience Institute, Université Libre de Bruxelles, Brussels, Belgium; Laboratoire de Neuropathologie Escourolle, Hôpital de la Pitié-Salpêtrière, Paris, France. Electronic address: Kunie.Ando@ulb.ac.be.
² Laboratoire de Neuropathologie Escourolle, Hôpital de la Pitié-Salpêtrière, Paris, France.
³ Laboratory of Histology, Neuroanatomy and Neuropathology, ULB Neuroscience Institute, Université Libre de Bruxelles, Brussels, Belgium.

PMID: 29496149
DOI: 10.1016/B978-0-444-63639-3.00021-9

Abstract

Recent innovations in tissue clearing and imaging technologies have enabled us to analyse biological systems directly in three-dimensions using thick samples. In this review, we discuss two of these recently reported tissue-clearing technologies (CLARITY and CUBIC) that are compatible with archival formalin-fixed human brain materials that have been fixed in formalin for a long period of time. We will discuss the pros and cons of these two technologies, examples of visualisation of Alzheimer neuropathological hallmarks and the exact protocols that we regularly use in the laboratory.

Keywords: 3D imaging; CLARITY; CUBIC; postmortem human brain tissues; tissue clearing.

MeSH terms

Brain / diagnostic imaging*
Histocytological Preparation Techniques / methods*
Humans
Imaging, Three-Dimensional*
Immunohistochemistry
Neuroimaging*