Objective: To investigate the reproductive and developmental toxicity of 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol in mice and to provide a basis for its risk assessment. Methods: The reproductive and developmental toxicity of 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol was tested using the screening method of chemicals with reproductive and developmental toxicity in "Chemical Testing Method" (SEPA). After five days of adaptive feeding, 120 specific pathogen-free healthy Kunming mice (male/female ratio=1:1) were orally administered 0 (control) , 146, 292, and 584 mg/kg 2- (2H-1, 2, 3-benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol for two weeks. One male mouse was mated with one female mouse in a single cage. The day on which a vaginal plug was observed was defined as gestation day 0 (GD0). The exposure for female mice was sustained to four days postpartum and the exposure for male mice was sustained for two weeks after mating. The body weight, food intake, body length, tail length, and sex ratio were recorded and the reproductive index was calculated. The reproductive organs were weighed and subjected to histopathological examination. Results: The 584 mg/kg group had significantly lower body weight at weeks 5 and 6 and food intake at week 6 in male mice, uterus weight and uterus/body weight ratio in female mice, and body weight, body length, and tail length on day 0 in offspring compared with the control group (all P<0.05). The 292 mg/kg group had significantly lower testis weight of male mice and food intake of female mice at gestational week 2 than the control group (both P<0.05). The 146 mg/kg group had significantly lower food intake of female mice at gestational week 2 than the control group (P<0.05) . Conclusion: For male and female Kunming mice, the no observed adverse effect levels of 2- (2H-1, 2, 3, -benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol are both 146 mg/kg.
目的： 对2-（2H-1，2，3-苯并三氮唑-2-基）-4-甲基-6-（2-甲基丙烯-2-基）苯酚（1）的生殖发育毒性进行研究，为进一步风险评价提供依据。 方法： 采用《化学品测试方法》（国家环保总局）中生殖和发育毒性筛选试验方法对化学物1进行生殖发育毒性试验研究。SPF级健康昆明种小鼠120只（雌、雄各60只），动物适应性饲养5 d后进行试验，按照0、146、292、584 mg/kg剂量经口灌胃染毒2周后，雄、雌（1:1）单笼交配，雌鼠阴栓阳性日为妊娠0 d，持续染毒至产后4 d；雄鼠交配后持续染毒2周。称量记录体重、进食量、身长、尾长、性别比，计算繁殖指数；称量生殖器官重量并进行病理组织学检查。 结果： 584 mg/kg剂量组雄性小鼠第5、6周体重、第6周进食量均低于对照组；雌性小鼠子宫重量和子宫/体重比值均低于对照组，差异具有统计学意义（P<0.05）；仔鼠第0天体重、身长、尾长均低于对照组，差异具有统计学意义（P<0.05）。292 mg/kg剂量组雄性小鼠睾丸重量低于对照组，差异具有统计学意义（P<0.05）。雌性小鼠妊娠后第2周进食量低于对照组，差异具有统计学意义（P<0.05）。146 mg/kg剂量组雌性小鼠妊娠第2周进食量低于对照组，差异具有统计学意义（P<0.05）。 结论： 化学物1对昆明种小鼠生殖发育毒性的NOAEL雌雄均为146 mg/kg。.
Keywords: 2- (2H-1, 2, 3, -benzotriazol-2-yl) -4-methyl-6- (2-methylpropen-2-yl) phenol; Mouse; Reproductive and developmental toxicity.