The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile

Sebastian Thieme; Alexander Holzbaur; Ralf Wiedemuth; Aline Binner; Katrin Navratiel; Konstantinos Anastassiadis; Sebastian Brenner; Cornelia Richter

doi:10.1371/journal.pone.0192437

The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile

PLoS One. 2018 Feb 28;13(2):e0192437. doi: 10.1371/journal.pone.0192437. eCollection 2018.

Authors

Sebastian Thieme¹, Alexander Holzbaur¹, Ralf Wiedemuth¹, Aline Binner¹, Katrin Navratiel¹, Konstantinos Anastassiadis², Sebastian Brenner^{1

3}, Cornelia Richter¹

Affiliations

¹ Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany.
² Stem Cell Engineering, BIOTEC, Technische Universitaet Dresden, Dresden, Germany.
³ Center for Regenerative Therapies Dresden, Technische Universitaet Dresden, Dresden, Germany.

Abstract

Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Transformed
Cell Polarity
Dendritic Cells / immunology*
Doxycycline / pharmacology*
Mice

Substances

Doxycycline

Grants and funding

This work was supported by the Collaborative Research Grant SFB-655 (DFG, SFB-655 project B6 to SB, project B1 to KA; www.dfg.de), the seed grants from the DFG-Center for Regenerative Therapies Dresden (to S.B.; www.crt-dresden.de) and the Saxon State Ministry for Higher Education, Research and the Arts (to C.R. and S.T.; www.sachsen.de/en/1218.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.