Silica nanoparticles with Tb(III)-centered luminescence decorated by Ag0 as efficient cellular contrast agent with anticancer effect

Svetlana V Fedorenko; Svetlana L Grechkina; Alsu R Mukhametshina; Anastasiya O Solovieva; Tatiana N Pozmogova; Svetlana M Miroshnichenko; Alexander Y Alekseev; Michael A Shestopalov; Kirill V Kholin; Irek R Nizameev; Asiya R Mustafina

doi:10.1016/j.jinorgbio.2018.02.002

Silica nanoparticles with Tb(III)-centered luminescence decorated by Ag⁰ as efficient cellular contrast agent with anticancer effect

J Inorg Biochem. 2018 May:182:170-176. doi: 10.1016/j.jinorgbio.2018.02.002. Epub 2018 Feb 8.

Affiliations

¹ A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation. Electronic address: svetlana@iopc.ru.
² A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
³ Research Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova srt., 630060 Novosibirsk, Russian Federation; Research Institute of Experimental and Clinical Medicine, 2 Timakova str., 630060 Novosibirsk, Russian Federation.
⁴ Research Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova srt., 630060 Novosibirsk, Russian Federation.
⁵ Research Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova srt., 630060 Novosibirsk, Russian Federation; Scientific Institute of Biochemistry, 2 Timakova st., 630060 Novosibirsk, Russian Federation.
⁶ Research Institute of Experimental and Clinical Medicine, 2 Timakova str., 630060 Novosibirsk, Russian Federation.
⁷ Research Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova srt., 630060 Novosibirsk, Russian Federation; Research Institute of Experimental and Clinical Medicine, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Nikolaev Institute of Inorganic Chemistry SB RAS, 3, Acad. Lavrentiev Ave., Novosibirsk 630090, Russian Federation.

PMID: 29486416
DOI: 10.1016/j.jinorgbio.2018.02.002

Abstract

The present work introduces composite luminescent nanoparticles (Ag⁰-Tb³⁺-SNs), where ultra-small nanosilver (4 ± 2 nm) is deposited onto amino-modified silica nanoparticles (35±6 nm) doped by green luminescent Tb(III) complexes. Ag⁰-Tb³⁺-SNs are able to image cancer (Hep-2) cells in confocal microscopy measurements due to efficient cell internalization, which is confirmed by TEM images of the Hep-2 cells exposed by Ag⁰-Tb³⁺-SNs. Comparative analysis of the cytotoxicity of normal fibroblasts (DK-4) and cancer cells (Hep-2) incubated with various concentrations of Ag⁰-Tb³⁺-SNs revealed the concentration range where the toxic effect on the cancer cells is significant, while it is insignificant towards the nonmalignant fibroblasts cells. The obtained results reveal Ag⁰-Tb³⁺-SNs as good cellular contrast agent able to induce the cancer cells death, which makes them promising theranostic in cancer diagnostics and therapy.

Keywords: Anticancer activity; Cellular uptake; Cytotoxicity; Lanthanide; Silica nanoparticles; Silver nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology
Cell Death / drug effects
Cell Line, Tumor
Contrast Media / chemistry*
Humans
Lanthanoid Series Elements / metabolism
Metal Nanoparticles / chemistry*
Microscopy, Confocal
Nanoparticles / chemistry*
Silicon Dioxide / chemistry*
Silver / chemistry

Substances

Antineoplastic Agents
Contrast Media
Lanthanoid Series Elements
Silver
Silicon Dioxide