Microparticle-based platform for point-of-care immunoassays

Teppo Salminen; Etvi Juntunen; Merja Lahdenranta; Iida Martiskainen; Sheikh M Talha; Kim Pettersson

doi:10.1016/j.ab.2018.02.021

Microparticle-based platform for point-of-care immunoassays

Anal Biochem. 2018 May 1:548:66-68. doi: 10.1016/j.ab.2018.02.021. Epub 2018 Feb 24.

Authors

Teppo Salminen¹, Etvi Juntunen², Merja Lahdenranta², Iida Martiskainen², Sheikh M Talha², Kim Pettersson²

Affiliations

¹ Department of Biotechnology, University of Turku, Turku, Finland. Electronic address: teppo.salminen@utu.fi.
² Department of Biotechnology, University of Turku, Turku, Finland.

PMID: 29486205
DOI: 10.1016/j.ab.2018.02.021

Abstract

There is a need for quantitative and sensitive, yet simple point-of-care immunoassays. We have developed a point-of-care microparticle-based immunoassay platform which combines the performance of a microtiter well-based assay with the usability of a rapid assay. The platform contained a separate reaction and detection chambers and microparticles for the solid-phase. Photoluminescent up-converting nanoparticles (UCNPs) were used as labels. The platform was tested with a cardiac troponin I assay, and a limit of detection of 19.7 ng/L was obtained. This study demonstrates the feasibility of developing point-of-care assays on the new platform for various analytes of interests.

Keywords: Cardiac troponin; Fluorescence; Microparticle; Nanoparticle; Point-of-care; Up-conversion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Immunoassay / methods
Nanoparticles / chemistry*
Point-of-Care Systems*
Troponin I / blood*

Substances

Troponin I