Álvarez-Yates, T and García-García, O. Effect of a hamstring flexibility program performed concurrently during an elite canoeist competition season. J Strength Cond Res 34(3): 838-846, 2020-The aim of this study was to determine the acute effect of an 8-week specific hamstring flexibility training on junior canoeist during the competition season. Sixteen canoeists participated, divided into 3 groups: 5 in experimental group 1 (EG1), 5 in experimental group 2 (EG2), and 6 in a control group. The EG1 underwent a supervised program consisted by simple static stretching, dynamic static stretching, and proprioceptive neuromuscular facilitation (PNF), whereas the EG2 performed a program without PNF and no supervision. Two measurements were performed to analyze the effect on the knee joint range of motion (ROM) and trunk flexion; neuromuscular and contractile properties in biceps femoris (BF) and semitendinosus (ST) muscles; and maximum power and average speed in lower limbs. A mixed-design factorial analysis of variance (p ≤ 0.05) was performed and the effect size was calculated. The results show significant improvements (p ≤ 0.05) in both legs for knee joint ROM and trunk flexion in EG1 (8.9 and 25.5%; 5.7 and 20.9% in the right and left leg, respectively) and EG2 (6.7 and 22%; 3.9 and 24.4%), only EG1 improved muscular stiffness in BF (27.4% left leg) and ST (20.5 and 24.3%), and lateral symmetry in BF (49%) without decreasing average speed in lower limbs. All improvements have a large effect size. In conclusion, it is suggested that a supervised hamstring flexibility program performed concurrently during a canoeist competition season can produce an increase in the knee joint ROM and trunk flexion, without negative repercussions in contractile and neuromuscular capacities, maximum power, and average velocity in lower limbs.