Characterization of endocrine disruption potentials of coastal sediments of Taean, Korea employing H295R and MVLN assays-Reconnaissance at 5years after Hebei Spirit oil spill

Xiaoshan Liu; Dawoon Jung; Kairu Zhou; Sangwoo Lee; Kiwan Noh; Jong Seong Khim; John P Giesy; Un Hyuk Yim; Won Joon Shim; Kyungho Choi

doi:10.1016/j.marpolbul.2017.11.055

Characterization of endocrine disruption potentials of coastal sediments of Taean, Korea employing H295R and MVLN assays-Reconnaissance at 5years after Hebei Spirit oil spill

Mar Pollut Bull. 2018 Feb:127:264-272. doi: 10.1016/j.marpolbul.2017.11.055. Epub 2017 Dec 9.

Authors

Xiaoshan Liu¹, Dawoon Jung², Kairu Zhou¹, Sangwoo Lee³, Kiwan Noh⁴, Jong Seong Khim⁵, John P Giesy⁶, Un Hyuk Yim⁷, Won Joon Shim⁷, Kyungho Choi⁸

Affiliations

¹ Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Guangdong, China.
² School of Public Health & Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea; Korea Environment Institute, Sejong, Republic of Korea.
³ School of Public Health & Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea; Korea Institute of Toxicology, Daejeon, Republic of Korea.
⁴ School of Public Health & Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea.
⁵ School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
⁶ Department of Veterinary Biomedical Sciences & Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.
⁷ Oil and POPs Research Group, Korea Institute of Ocean Science and Technology (KIOST), Geoje, Republic of Korea.
⁸ School of Public Health & Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea. Electronic address: kyungho@snu.ac.kr.

PMID: 29475662
DOI: 10.1016/j.marpolbul.2017.11.055

Abstract

Endocrine disrupting potentials were assessed for sediment samples collected near Hebei Spirit oil spill (HSOS) site, between December 2007 and January 2012. For comparison, major crude oil (CO) of HSOS, or its weathered form were assessed. Both raw extracts (REs) and their fractionated samples were tested using H295R and MVLNluc bioassays. In H295R cells, REs of crude and weathered oil (WO), and nine of 14 sediments significantly increased E2 levels, which were correlated with the concentrations of PAHs. Steroidogenic disruption potentials of the sediments generally decreased over time. Among silica fractions of all REs, aromatic hydrocarbons (F2) and polar compounds (F3) caused greater E2 levels. While, in MVLN cell bioassay, only three of 14 sediment REs showed estrogen receptor binding potencies, and no temporal trend was observed. In conclusion, oil spill can cause endocrine disruption in the affected ecosystem through steroidogenic alteration for years, and such potencies attenuate over time.

Keywords: Endocrine disrupting potential; H295R; Hebei Spirit oil spill; MVLN.

MeSH terms

Biological Assay
Cell Line, Tumor
Ecosystem
Endocrine Disruptors / analysis
Endocrine Disruptors / toxicity*
Environmental Monitoring
Estradiol / metabolism
Geologic Sediments* / analysis
Humans
Petroleum / analysis
Petroleum / toxicity
Petroleum Pollution / adverse effects*
Polycyclic Aromatic Hydrocarbons / analysis
Polycyclic Aromatic Hydrocarbons / toxicity*
Republic of Korea
Water Pollutants, Chemical / analysis
Water Pollutants, Chemical / toxicity*
Weather

Substances

Endocrine Disruptors
Petroleum
Polycyclic Aromatic Hydrocarbons
Water Pollutants, Chemical
Estradiol