Effect of Laser Therapy on Defensins' Gene Expression in TR146 Epithelial Cell Line

Protein Pept Lett. 2018;25(4):326-329. doi: 10.2174/0929866525666180221123249.

Abstract

Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects.

Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3.

Materials and method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform.

Results and conclusion: In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β -defensins production immediately after treatment, while its efficacy decreases over time.

Keywords: Innate immunity; gene expression; laser therapy; oral mucositis; real time PCR; β-defensins..

MeSH terms

  • Cell Survival
  • Epithelial Cells / metabolism*
  • Gene Expression
  • Humans
  • Laser Therapy*
  • Mouth Mucosa / metabolism
  • Pilot Projects
  • RNA, Messenger / metabolism
  • beta-Defensins / genetics
  • beta-Defensins / metabolism*

Substances

  • DEFB1 protein, human
  • DEFB103A protein, human
  • RNA, Messenger
  • beta-Defensins