Comprehensive virome analysis reveals the complexity and diversity of the viral spectrum in pediatric patients diagnosed with severe and mild hand-foot-and-mouth disease

Chunhua Wang; Shuaifeng Zhou; Wanhua Xue; Liang Shen; Wei Huang; Yi Zhang; Xuguang Li; Junzhi Wang; Hong Zhang; Xuejun Ma

doi:10.1016/j.virol.2018.02.004

Comprehensive virome analysis reveals the complexity and diversity of the viral spectrum in pediatric patients diagnosed with severe and mild hand-foot-and-mouth disease

Virology. 2018 May:518:116-125. doi: 10.1016/j.virol.2018.02.004. Epub 2018 Feb 19.

Authors

Chunhua Wang¹, Shuaifeng Zhou², Wanhua Xue³, Liang Shen⁴, Wei Huang², Yi Zhang⁴, Xuguang Li⁵, Junzhi Wang⁶, Hong Zhang⁷, Xuejun Ma⁸

Affiliations

¹ National Institutes for Food and Drug Control, Beijing 100050, China; Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
² Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, 410005, China.
³ Dezhou People's Hospital, Dezhou, Shandong, 253056, China.
⁴ Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
⁵ Biologics and Genetic Therapies Directorate, Health Canada, Tunney's Pasture, Ottawa, AL 2201C, Canada.
⁶ National Institutes for Food and Drug Control, Beijing 100050, China. Electronic address: wangjz@nifdc.org.cn.
⁷ Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, 410005, China. Electronic address: 931525821@qq.com.
⁸ Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: maxj@ivdc.chinacdc.cn.

PMID: 29471150
DOI: 10.1016/j.virol.2018.02.004

Abstract

The management of hand-foot-and-mouth disease(HFMD) epidemic is difficult due to the frequent emergence of non-EV71 and non-CVA16 enteroviruses and some cases testing negative for HFMD-associated causative agents. To clarify the virus spectrum of mild and severe HFMD, a comprehensive virome analysis of 238 samples was performed using next-generation sequencing (NGS). The data revealed total thirteen mammalian- and plant- virus families and diverse viral populations including enteroviruses, common respiratory viruses, diarrhea-related viruses, plant viruses and anelloviruses. A total of 18 viruses from 7 virus families were identified in severe cases, versus 37 viruses from 12 virus families in mild cases. Moreover, complicated mixed-infections of enteroviruses with common respiratory viruses were mainly found in severe cases(P = 0.013), while diarrhea-related viruses were mainly found in mild cases(P < 0.001). This study provides the preliminary understanding of viromes both in mild and severe cases, which may benefit the detection of etiologic agents and prevention of HFMD.

Keywords: Complexity; Diversity; Mild HFMD; Next-generation sequencing; Severe HFMD; Virome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Child
Child, Preschool
China / epidemiology
Enterovirus A, Human / genetics*
Feces / virology
Genetic Variation
Hand, Foot and Mouth Disease / epidemiology
Hand, Foot and Mouth Disease / pathology*
Hand, Foot and Mouth Disease / virology*
Humans
Infant
Male
Phylogeny