Background: The objective of the study was to investigate the changes in amino acid (AAs) values in premature infants.
Methods: A total of 2159 premature and/or low birth weight infants were recruited for this study. They were divided into three groups: premature infants with normal birth weight (G1), simple low birth weight infants (G2) and premature combined with low birth weight infants (G3). The tandem mass spectrometry technique was used to detect the levels of 11 AAs in neonatal blood.
Results: Compared with normal babies, there were eight and five AAs that significantly changed in G1 and G2, respectively. It was worth noting that the changes greatly exacerbated when the babies were both premature and of low birth weight. All the levels of AAs demonstrated significant changes in G3 compared with the normal control group (G4). With the increase in gestational age, the AAs in premature infants tended to the levels in normal newborns. Meanwhile, there was a correlation between AAs and birth weight. Four AAs significantly changed with the increase in body weight. Among normal newborns, the levels of AAs in girls were significantly higher than in boys. However, if the newborns were premature or had low birth weight, the differences between AA values and sexual distinction would decrease. In the end, we established the specific reference ranges of AAs for premature and/or low birth weight infants.
Conclusions: There were significant differences in AAs in the premature and/or low birth weight infants. Gestational age and birth weight were two important factors inflecting the AAs metabolism.
Keywords: amino acids; low birth weight; newborn screening; premature; tandem mass spectrometry.