Objective: To explore the anti-tumor effect of 17XL strains of Plasmodium yoelii (P.y) infection on melanoma in mice.
Methods: B16F10 tumor cells were axillarilly injected into the right flank of 20 C57BL/6 mice to establish tumor-bearing mouse models. The next day, the mice were randomly divided into a P.y infection group and control group, 10 mice each group. Each mouse of the P.y infection group was intraperitoneally injected with 1×106 red blood cells including 20% P.y infection red blood cells, and each one of the control group were intraperitoneally injected with 1×106 normal red blood cells of C57BL/6 mice. The time of tumor formation of the mice in the two groups was observed and the tumor volumes were measured.
Results: The time of tumor formation in the P.y infection group［ (11.30 ± 0.21) d］was significantly later than that in the control group ［ (10.40 ± 0.22) d］ (P < 0.05). From the tumors could be accurately measured to the study end point, both the tumors of mice in the two groups were growing, and the tumor volumes of mice in the P.y infection group were significantly less than those in the control group at each time point (all P < 0.05). The growth rate of tumors in the P.y infection group ［ (71.10 ± 6.29) mm3/d］ was significantly slower than that in the control group ［ (302.80 ± 49.94) mm3/d］ (P < 0.05), and the growth rates of tumors everyday in the P.y infection group were significantly slower than those in the control group (all P < 0.05).
Conclusions: The P.y infection can delay the occurrence of tumor and inhibit the growth of melanoma.
[摘要]目的 观察约氏疟原虫17XL虫株感染对小鼠黑色素瘤的抑制作用。方法 取C57BL/6小鼠20只, 腋下注射传 代培养的B16F10黑色素瘤细胞; 次日, 将小鼠随机分为约氏疟原虫 (P.y) 感染组和对照组2组, 10只/组; P.y 感染组小鼠 每只腹腔注射1×106个含虫红细胞率为20%的小鼠红细胞; 对照组小鼠腹腔注射等量的C57BL/6小鼠正常红细胞。观察 两组小鼠黑色素瘤出瘤时间并测量肿瘤的体积。结果 P.y 感染组小鼠黑色素瘤出瘤时间为注射黑色素瘤细胞后 (11.30 ± 0.21) d, 晚于对照组［ (10.40 ± 0.22) d］ (P < 0.05); 自可精确测量瘤体起至实验终点, 2组小鼠肿瘤均不断增长, P.y 感染组瘤体体积均显著小于对照组 (P 均< 0.05); P.y 感染组瘤体生长速度为 (71.10 ± 6.29) mm3/d, 明显慢于对照组 ［ (302.80 ± 49.94) mm3/d］ (P < 0.05), 且P.y感染组肿瘤每日生长速度亦明显慢于对照组 (P均< 0.05)。结论 约氏疟原 虫感染可以延缓肿瘤出瘤时间, 且对小鼠黑色素瘤瘤体的增长具有明显的抑制作用。.
Keywords: Anti-tumor effect; Infection; Melanoma; Mouse; Plasmodium yoelii.