Paucibacter aquatile sp. nov. isolated from freshwater of the Nakdong River, Republic of Korea

Arch Microbiol. 2018 Aug;200(6):877-882. doi: 10.1007/s00203-018-1494-2. Epub 2018 Feb 21.

Abstract

A Gram-negative, aerobic, motile, and rod-shaped bacterial strain designated CR182T was isolated from freshwater of the Nakdong River, Republic of Korea. Optimal growth conditions for this novel strain were found to be: 25-30 °C, pH 6.5-8.5, and 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicates that the strain CR182T belongs to type strains of genus Paucibacter. Strain CR182T showed 98.0% 16S rRNA gene sequence similarity with Paucibacter oligotrophus CHU3T and formed a robust phylogenetic clade with this species. The average nucleotide identity value between strain CR182T and P. oligotrophus CHU3T was 78.4% and the genome-to-genome distance was 22.2% on average. The genomic DNA G+C content calculated from the genome sequence was 66.3 mol%. Predominant cellular fatty acids of strain CR182T were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) (31.2%) and C16:0 (16.0%). Its major respiratory quinine was ubiquinone Q-8. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified phospholipids. Its genomic DNA G+C content was 66.3%. Based on data obtained from this polyphasic taxonomic study, strain CR182T represents a novel species belonging to genus Paucibacter, for which a name of P. aquatile sp. nov. is proposed. The type strain is CR182T (= KCCM 90284T = NBRC 113032T).

Keywords: Novel species; Paucibacter aquatile; Polyphasic taxonomy.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Burkholderiales / classification
  • Burkholderiales / genetics
  • Burkholderiales / isolation & purification*
  • Burkholderiales / metabolism
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Phospholipids / chemistry
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rivers / microbiology*
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S