Comparative evaluation of GeneXpert MTB/RIF and multiplex PCR targeting mpb64 and IS6110 for the diagnosis of pleural TB

Future Microbiol. 2018 Mar:13:407-413. doi: 10.2217/fmb-2017-0147. Epub 2018 Feb 21.

Abstract

Aim: Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test.

Methods: We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (n = 78) of pleural TB patients and non-TB controls.

Results: The sensitivities of 89.6 and 33.3%, and specificities of 96.7 and 100%, were observed with M-PCR and Xpert assay, respectively.

Conclusion: M-PCR showed superiority over Xpert assay and may facilitate an efficient diagnosis of pleural TB.

Keywords: GeneXpert; IS6110; Mycobacterium tuberculosis; mpb64; multiplex PCR; paucibacillary; pleural TB; pleural fluid; sensitivity; specificity.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Bacterial Proteins / genetics
  • Diagnostic Tests, Routine / methods
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • Pleural Effusion / microbiology*
  • Sensitivity and Specificity
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology
  • Young Adult

Substances

  • Bacterial Proteins