Promoter Variation and Gene Expression of mcr-1-Harboring Plasmids in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese Hospital

Antimicrob Agents Chemother. 2018 Apr 26;62(5):e00018-18. doi: 10.1128/AAC.00018-18. Print 2018 May.

Abstract

Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.

Keywords: colistin; gene expression; genomics; plasmids; promoter.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • China
  • Colistin / pharmacology
  • Drug Resistance, Bacterial / genetics*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Hospitals
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics
  • Plasmids / genetics*
  • Promoter Regions, Genetic / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Colistin