Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesiclesassociated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urine extracellular vesicles and demonstrated diagnostic potential of their miRNA cargo. In this study, we have performed a comparative analysis of the expression of 84 miRNA in paired samples of urine microvesicles and clarified urine from healthy men, patients with benign hyperplasia and cancer of the prostate using miRCURY LNA miRNA qPCR Panels. Subsets of miRNAs with differences in expression between the fractions of the urine were found in all three groups. Two groups of miRNA were identified based on the patterns of their differential expression. They regulate several key signaling pathways associated with prostate cancer development.
Pri rake predstatel'noĭ zhelezy v moche obnaruzhivaiutsia opukholevospetsifichnye biopolimery, v tom chisle sviazannye v kompleksy s belkami i pogruzhennye vo vnekletochnye vezikuly miRNK, kotorye mogut byt' ispol'zovany v kachestve biomarkerov onkologicheskikh zabolevaniĭ. Ranee nami byli okharakterizovany vezikuly mochi i pokazana diagnosticheskaia perspektivnost' soderzhashchikhsia v nikh miRNK. V nastoiashchem issledovanii byl proveden sravnitel'nyĭ analiz 84 miRNK v parnykh obraztsakh mikrovezikul i supernatanta mochi zdorovykh donorov, patsientov s dobrokachestvennoĭ giperplazieĭ i rakom predstatel'noĭ zhelezy na platforme miRCURY LNA miRNA qPCR Panels. Vo vsekh gruppakh donorov naĭdeny nabory miRNK, uroven' kotorykh dostoverno razlichalsia mezhdu fraktsiiami. Vydeleny 2 gruppy miRNK, issledovany spektry ikh misheneĭ i pokazano ikh uchastie v riade signal'nykh puteĭ, v tom chisle sviazannykh s patogenezom RPZh.
Keywords: exosomes; miRNA; microvesicles; prostate cancer; urine.