The objective of this prospective experimental clinical trial was to evaluate intraoral burning sensation in response to heat-cured and self-cured acrylic resin denture base material and carried out in the department of Prosthodontics of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh from February 2014 to September 2015. Forty eight patients were evaluated in this study; of them 35(72.92%) patients were male and 13(27.08%) patients were female with age range from 24 to 70 years. In this study 48 patients were evaluated in term of burning sensation, of them 24 patients were provided with heat-cured acrylic resin denture and were considered as Group A and 24 patients were provided with self-cured acrylic resin denture and were considered as Group B. In Group A 14(58.4%) at 1st week, 2(8.4%) at 1st month, 10(41.8%) at 3rd month and 5(21.0%) at 6th month developed burning sensation. On the other hand the values were 18(75.0%), 22(91.6%), 14(58.2%) and 2(8.4%) at 1stweek, 1st month, 3rd month, and 6th month respectively. Chi-square test was done for statistical analysis and p value was <0.05. So, results were statistically significant.