Controlled release of mitomycin C from PHEMAH-Cu(II) cryogel membranes

Artif Cells Nanomed Biotechnol. 2018;46(sup1):946-954. doi: 10.1080/21691401.2018.1439840. Epub 2018 Feb 19.

Abstract

Molecular imprinting technique was used for the preparation of antibiotic and anti-neoplastic chemotherapy drug (mitomycin C) imprinted cryogel membranes (MMC-ICM). The membranes were synthezied by using metal ion coordination interactions with N-methacryloyl-(l)-histidine methyl ester (MAH) functional monomer and template molecules (i.e. MMC). The 2-hydroxyethyl methacrylate (HEMA) monomer and methylene bisacrylamide (MBAAm) crosslinker were used for the preparation of mitomycin C imprinted cryogel membranes by radical suspension polymerization technique. The imprinted cryogel membranes were characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) and swelling degree measurements. Cytotoxicity of MMC-ICMs was investigated using mouse fibroblast cell line L929. Time-dependent release of MMC was demonstrated within 150 h from cryogel membranes. Cryogels demonstrated very high MMC loading efficiency (70-80%) and sustained MMC release over hours.

Keywords: Mitomycin C; controlled release; cryogel membrane; molecular imprinting.

MeSH terms

  • Cell Line
  • Copper / chemistry*
  • Cryogels / chemistry*
  • Delayed-Action Preparations
  • Histidine / analogs & derivatives*
  • Histidine / chemistry
  • Kinetics
  • Membranes, Artificial*
  • Mitomycin / chemistry*
  • Models, Molecular
  • Molecular Conformation
  • Molecular Imprinting
  • Polymerization
  • Polymethacrylic Acids / chemistry*

Substances

  • Cryogels
  • Delayed-Action Preparations
  • Membranes, Artificial
  • Polymethacrylic Acids
  • poly(hydroxyethyl methacrylate-N-methacryloyl-histidine methyl ester)
  • Histidine
  • Mitomycin
  • Copper