Tracking mitochondrial pH fluctuation during cell apoptosis with two-photon phosphorescent iridium(iii) complexes

Kangqiang Qiu; Libing Ke; Xuepeng Zhang; Yukang Liu; Thomas W Rees; Liangnian Ji; Jiajie Diao; Hui Chao

doi:10.1039/c8cc00299a

Tracking mitochondrial pH fluctuation during cell apoptosis with two-photon phosphorescent iridium(iii) complexes

Chem Commun (Camb). 2018 Mar 7;54(19):2421-2424. doi: 10.1039/c8cc00299a. Epub 2018 Feb 19.

Authors

Kangqiang Qiu¹, Libing Ke¹, Xuepeng Zhang², Yukang Liu¹, Thomas W Rees¹, Liangnian Ji¹, Jiajie Diao³, Hui Chao⁴

Affiliations

¹ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, P. R. China. ceschh@mail.sysu.edu.cn.
² Lab of Computational and Drug Design, Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, P. R. China.
³ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. jiajie.diao@uc.edu.
⁴ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, P. R. China. ceschh@mail.sysu.edu.cn and MOE Key Laboratory of Theoretical Organic Chemistry and Functional Molecule, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 400201, P. R. China.

PMID: 29457169
DOI: 10.1039/c8cc00299a

Abstract

Two iridium(iii) complexes with ligands containing morpholine groups were used to track mitochondrial pH fluctuation during cell apoptosis as their emissive intensities linearly increased with increase in pH values.

MeSH terms

Apoptosis / physiology*
Coordination Complexes / chemical synthesis
Coordination Complexes / chemistry
Coordination Complexes / radiation effects*
Coordination Complexes / toxicity
HeLa Cells
Humans
Hydrogen-Ion Concentration
Iridium / chemistry*
Ligands
Light
Luminescence
Luminescent Agents / chemical synthesis
Luminescent Agents / chemistry
Luminescent Agents / radiation effects*
Luminescent Agents / toxicity
Mitochondria / metabolism*
Morpholines / chemical synthesis
Morpholines / chemistry
Morpholines / radiation effects
Morpholines / toxicity
Spheroids, Cellular

Substances

Coordination Complexes
Ligands
Luminescent Agents
Morpholines
Iridium