Abstract
Investigation of the MeOH extract of Betula schmidtii twigs resulted in the isolation and identification of three new triterpenoids (1-3), along with ten known ones (4-13). The structures of new compounds (1-3) were elucidated by spectroscopic methods, including 1D, 2D NMR (1H and 13C NMR, COSY, HSQC, HMBC, and NOESY), HR-MS, and chemical methods. All the isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines. Compound 11 exhibited potent cytotoxic activities against four cell lines, and compounds 5 and 13 significantly induced nerve growth factor secretion in a C6 rat glioma cell line. Their anti-inflammatory effects were also assessed by measuring nitric oxide production in lipopolysaccharide-activated BV-2 cells. Compounds 7 and 12 displayed potent inhibition of nitric oxide production, without significant cell toxicity.
Keywords:
Anti-inflammation; Betula schmidtii; Betulaceae; Cytotoxicity; NGF regulation; Triterpenes.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Betula / chemistry*
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Cell Line
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Humans
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Lipopolysaccharides / antagonists & inhibitors
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Lipopolysaccharides / pharmacology
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Molecular Structure
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Nerve Growth Factor / antagonists & inhibitors
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Nerve Growth Factor / metabolism
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis
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Plant Stems / chemistry
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Structure-Activity Relationship
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Triterpenes / chemistry
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Triterpenes / isolation & purification
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Triterpenes / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antineoplastic Agents, Phytogenic
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Lipopolysaccharides
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Triterpenes
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Nitric Oxide
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Nerve Growth Factor