Is it photosensitization or photodegradation when UV-B irradiation is combined with BDE-47? Evidence from the growth and reproduction changes of rotifer Brachionus plicatilis

Abstract

Ecotoxicological methods were applied in the present study, and the marine rotifer Brachionus plicatilis was used as the toxic endpoint to depict what occurred when 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) was combined with solar ultraviolet-B radiation (UV-B). B. plicatilis was exposed to three different combination methods of BDE-47 and UV-B at an equal toxicity ratio, including normal rotifer co-cultured with UV-B-irradiated BDE-47 (known as Method I), UV-B-irradiated rotifer co-cultured with BDE-47 exposure (known as Method II) and normal rotifer co-cultured with the simultaneous addition of BDE-47 and UV-B irradiation (known as Method III). Acute and chronic experiments were preformed to determine the toxicity differentiation according to the growth and reproduction changes in the rotifer. Twenty-four-hour acute experiments showed that the modes of three combined methods changed from antagonism to additive, to synergistic with the concentration/dose increment, and the contribution rates of Method I and Method II to Method III were calculated by approximately 40.4% and 59.6%, respectively. Chronic exposure to either the single stressor or the combination of stressors inhibited the growth and reproduction of the rotifer, demonstrating the inhibition of the population growth rate and the decrease in the larvae production. Three combined groups presented more serious damages compared to groups with single stress exposure, and the ascending sequence of toxicity was Method I<Method II<Method III. A higher bioaccumulation of BDE-47 was found in all combined groups than BDE-47 single stress group, and bioconcentration factor (BCF) general ranked Method II<Method I<Method III. Moreover, BDE-28, photodegradation production of BDE-47, were found in groups preformed Method I and III. We thus speculated that the toxicity enhancement when BDE-47 was combined with UV-B was mainly due to photosensitization and photodegradation, and the photosensitization might be more noxious to the growth and reproduction of the rotifer.

Keywords: 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47); Brachionus plicatilis; Joint effect; Photodegradation; Photosensitization; Ultraviolet-B h(UV-B) irradiation.