A double layered plasmonic device based on transferring technique with polystyrene nano-beads is analyzed and demonstrated to increase the sensing characteristics of plasmonic sensor system. The double layered plasmonic devices are calculated using the three-dimensional finite-difference time-domain method for the width and thickness of the nano-hole structures. The double layered plasmonic devices with different diameters of the Au nano-hole are fabricated by transferring method with commercially available chloromethyl latex with a diameter of 0.42 μm. The optimum sensing characteristic of the proposed plasmonic device is obtained with the film and the hole thickness of 15 and 15 nm in the 246 nm wide nano-hole size. The best sensitivity of the proposed plasmonic sensor is 67.7 degree/RIU when the sensitivity of the conventional plasmonic sensor is 42.2 degree/RIU.