Generation of induced pluripotent stem cell line (ZZUi004-A) from urine sample of a patient with spinocerebellar ataxia type 3

Yanlin Wang; Changhe Shi; Zhilei Wang; Huifang Sun; Zhihua Yang; Fan Zhang; Yutao Liu; Han Liu; Chenyang Jiang; Shoutao Zhang; Yuming Xu; Xuejun Wen

doi:10.1016/j.scr.2018.01.027

Generation of induced pluripotent stem cell line (ZZUi004-A) from urine sample of a patient with spinocerebellar ataxia type 3

Stem Cell Res. 2018 Apr:28:71-74. doi: 10.1016/j.scr.2018.01.027. Epub 2018 Jan 31.

Authors

Yanlin Wang¹, Changhe Shi¹, Zhilei Wang¹, Huifang Sun¹, Zhihua Yang¹, Fan Zhang², Yutao Liu¹, Han Liu¹, Chenyang Jiang¹, Shoutao Zhang³, Yuming Xu⁴, Xuejun Wen⁵

Affiliations

¹ Department of Neurology, The First affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China.
² Department of Geriatrics, The Second affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan, China.
³ School of life sciences, Zhengzhou University, Zhengzhou, Henan 450001, China.
⁴ Department of Neurology, The First affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China. Electronic address: xuyuming@zzu.edu.cn.
⁵ Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA, USA; Shanghai East Hospital, The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200120, China. Electronic address: xwen@vcu.edu.

PMID: 29444500
DOI: 10.1016/j.scr.2018.01.027

Abstract

Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the region of the ATXN3 gene. The main feature of SCA3 is progressive ataxia, which affects balance, gait, and speech. Urine cells (UCs) of a SCA3 patient were successfully translated to induced pluripotent stem cells (iPSCs) by using the Sendai virus delivery system. ZZUi004-A cell line may provide a robust platform for further study of SCA3 pathogenesis as well as drug testing and gene therapy research.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Female
Humans
Induced Pluripotent Stem Cells / cytology*
Machado-Joseph Disease / pathology*
Machado-Joseph Disease / urine*
Microsatellite Repeats / genetics
Mycoplasma / isolation & purification