A novel dicyanoisophorone based red-emitting fluorescent probe with a large Stokes shift for detection of hydrazine in solution and living cells

Hongshui Lv; Haiyan Sun; Shoujuan Wang; Fangong Kong

doi:10.1016/j.saa.2018.02.026

A novel dicyanoisophorone based red-emitting fluorescent probe with a large Stokes shift for detection of hydrazine in solution and living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5:196:160-167. doi: 10.1016/j.saa.2018.02.026. Epub 2018 Feb 8.

Authors

Hongshui Lv¹, Haiyan Sun², Shoujuan Wang², Fangong Kong³

Affiliations

¹ School of Paper-making and Botanical Resources Engineering, Key Lab of Pulp and Paper Science & Technology, Ministry of Education (Shandong Province), Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. Electronic address: sdlhs@qlu.edu.cn.
² School of Paper-making and Botanical Resources Engineering, Key Lab of Pulp and Paper Science & Technology, Ministry of Education (Shandong Province), Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
³ School of Paper-making and Botanical Resources Engineering, Key Lab of Pulp and Paper Science & Technology, Ministry of Education (Shandong Province), Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. Electronic address: kfg@qlu.edu.cn.

PMID: 29444498
DOI: 10.1016/j.saa.2018.02.026

Abstract

A novel dicyanoisophorone based fluorescent probe HP was developed to detect hydrazine. Upon the addition of hydrazine, probe HP displayed turn-on fluorescence in the red region with a large Stokes shift (180nm). This probe exhibited high selectivity and high sensitivity to hydrazine in solution. The detection limit of HP was found to be 3.26ppb, which was lower than the threshold limit value set by USEPA (10ppb). Moreover, the probe was successfully applied to detect hydrazine in different water samples and living cells.

Keywords: Cell imaging; Fluorescent probe; Hydrazine; Large Stokes shift; Red emission.

MeSH terms

Cyclohexanones / chemistry
Cytological Techniques / methods*
Dimethyl Sulfoxide
Drug Stability
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Hydrazines / analysis*
Hydrazines / metabolism
Hydrogen-Ion Concentration
Isocyanates / chemistry*
Microscopy, Confocal
Microscopy, Fluorescence

Substances

Cyclohexanones
Fluorescent Dyes
Hydrazines
Isocyanates
hydrazine
isophorone
isophorone diisocyanate
Dimethyl Sulfoxide